External lysis of Escherichia coli by a bacteriophage endolysin modified with hydrophobic amino acids
نویسندگان
چکیده
منابع مشابه
Lysis of Escherichia coli cells induced by bacteriophage T4.
Structural changes in the envelope of Escherichia coli cells accompanying their lysis from without by bacteriophage T4 have been studied. The hypothesis concerning the role of collapse of membrane potential and formation of periplasmic vesicles in the process of lysis from without has been advanced.
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The Staphylococcus aureus bacteriophage phi11 endolysin has two peptidoglycan hydrolase domains (endopeptidase and amidase) and an SH3b cell wall-binding domain. In turbidity reduction assays, the purified protein can lyse untreated staphylococcal mastitis pathogens, Staphylococcus aureus and coagulase-negative staphylococci (Staphylococcus chronogenes, Staphylococcus epidermidis, Staphylococcu...
متن کاملA study of lysis in bacteriophage-infected Escherichia coli.
Several hypotheses have been proposed to explain the poorly understood mechanism by which virulent bacteriophage induces lysis in bacteria (Hershey and Bronfenbrenner, 1952; Luria, 1953). It has been suggested that lysis is caused by: 1) the accumulation of an overwhelming amount of phage within the cell (d'Herelle, 1926); 2) the possession by the phage of a lytic enzyme (Sertic, 1929; Anderson...
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Escherichia coli cells are shown to be attracted to the l-amino acids alanine, asparagine, aspartate, cysteine, glutamate, glycine, methionine, serine, and threonine, but not to arginine, cystine, glutamine, histidine, isoleucine, leucine, lysine, phenylalanine, tryptophan, tyrosine, or valine. Bacteria grown in a proline-containing medium were, in addition, attracted to proline. Chemotaxis tow...
متن کاملCharacterization of oxygen-dependent lysis of Escherichia coli cells infected by bacteriophage T4.
Infection of Escherichia coli cells by T4 phage at a multiplicity of infection (MOI) of 0.01 caused inhibition of cell lysis for up to 4 h. Such cells grown under aerobic condition were lysed by external stimuli such as cold shock, osmotic shock or addition of toxic substances, e.g., carbonyl cyanide m-chlorophenylhydrazone (CCCP). However, the effects of these external stimuli were reduced by ...
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ژورنال
عنوان ژورنال: AMB Express
سال: 2019
ISSN: 2191-0855
DOI: 10.1186/s13568-019-0838-x